Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection

Replicative virus shedding in the respiratory tract of patients with Middle East respiratory syndrome coronavirus infection

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Background: Information on the duration of replicative Middle East respiratory syndrome coronavirus (MERS-CoV) shedding is important for infection control.The detection of MERS-CoV sub-genomic mRNAs indicates that the virus is replicative.This study examined the duration for detecting MERS-CoV sub-genomic mRNA compared with genomic RNA in diverse respiratory specimens.Methods: Upper canine spectra kc 3 intranasal single dose and lower respiratory samples were obtained from 17 MERS-CoV-infected patients.MERS-CoV sub-genomic mRNA was detected by reverse transcription PCR (RT-PCR) and MERS-CoV genomic RNA by real-time RT-PCR.

Results: In sputum and transtracheal aspirate, sub-genomic mRNA was detected for up to 4 weeks after symptoms developed, which correlated with the detection of genomic RNA.In oropharyngeal and nasopharyngeal swab specimens, the detection of sub-genomic mRNA and genomic RNA did not correlate.Conclusions: These findings suggest that MERS-CoV does not replicate well in the upper respiratory iphone 14 price texas tract.Keywords: Coronavirus infections, RNA, Messenger, Respiratory system.